Glass and other Sharp Injuries in the Laboratory

The purpose of this research safety guidance is to prevent and/or minimize broken glass injuries while handling laboratory glassware through:

  1. awareness of personal protective measures, including the use of slip-resistant and cut-resistant gloves while handling glassware;
  2. safe disposal of both broken and unbroken glassware, pipettes and vials, etc., in sharps container to prevent injury to those handling waste containers; and
  3. the online submission of the First Report of Injury (FROI) form for reporting injuries; this facilitates compliance reporting as well as follow-up evaluations and corrective actions to prevent future incidents.


This guidance applies to all research and teaching labs, manufacturing units, and staff and students who handle glassware in lab experiments, e.g., making media, reagent preparation, synthesis, analysis, vacuum techniques, and glassware cleaning. Additional site specific procedures may be incorporated to meet the needs of individual work areas, core facilities, process labs, and manufacturing units in the university. Potential Risk

Potential Risk

  • Broken glass and other sharps are physical hazards.
  • Broken glass also has the potential to be a health hazard if it is contaminated with toxic chemicals, blood, or infectious substances which may enter the body through a cut or puncture.

Broken glass penetrates the skin and causes lacerations just like sharp, needles, and razors.

The most likely person to be injured from mishandling of glassware is the user. However, anyone in the vicinity of imploding glass is likely to be hurt, so it is important for everyone to wear their personal protective equipment (PPE). The probability of being injured by broken or breaking glass is ‘LIKELY’ and the potential severity of injury from a pressurized or vacuum glass apparatus incident is ‘SEVERE TO EXTREME.’ By implementing safety controls and using PPE, the potential risks associated with glassware handling can be reduced.  Please see the table below.

‘LIKELY’ and the potential severity of injury from a pressurized or vacuum glass apparatus incident is ‘SEVERE TO EXTREME.’ By implementing safety controls and using PPE, the potential risks associated with glassware handling can be reduced.  Please see the table below.

Specialized glassware, including Schlenk line tubes, separatory funnels, vials, desiccators, autoclavable tubing/glassware, micro-fabrication and thin film deposition devices, laser optics, etc., are prone to breakage from hairline cracks and from dropping the item. Wear slip-resistant or cut-resistant gloves when handling glass to prevent cuts, abrasions, and skin puncture.

Type of Glassware Injury Safety Control Recommendations for Risk Reduction Example Process/Activities Potential Risk
Injury from imploding glass or flying glass.
(Vacuum apparatus)
  • Tape vacuum flasks and vacuum desiccators with electrical tape.
  • Keep cryogenic vacuum flasks wrapped with Nylon or other polymer based plastic mesh (view example).
  • Wrap glass desiccators with friction or electrical tape in a grid pattern, leaving the contents visible; this will guard against flying glass in case of implosion.
  • Wear a face shield or safety glasses with side shields, appropriate gloves, and lab coat.
  • Educate researchers in vacuum techniques.

Consider other factors that could possibly reduce risks, such as: Use low intensity vacuum devices, use smaller flasks, assure vacuum is released at the vacuum pump before removing vacuum flask/glassware, and make sure glassware under vacuum is not located where it could be bumped or struck.

Working with vacuum apparatus. HIGH
Cuts when forcing glass tubing or pipettes onto rubber stoppers
  • Use glycerin as a lubricant.
  • Wear cut-resistant gloves and safety glasses with side shields.
Working with glass tubes or Pasteur pipettes. Moderate Plus
Cuts caused by breaking inlet/outlet stems from reflux condensers or desiccator stems
  • Dip the end of Tygon tubing into a solvent such as acetone and gently insert onto glass stems.
  • Wear cut-resistant gloves, safety glasses with side shields, and lab coat.
Assembling/disassembling condensers, or holding the stem to open or close desiccators. Moderate
Chemical or other hazardous material exposure caused by sharps injuries from contaminated glassware
  • Develop and implement hazardous chemical or hazardous material-specific SOPs.
  • Work within a fume hood or appropriate engineering control.
  • Wear gloves, safety glasses with side shields, and lab coat.
  • Wear a face shield or safety goggles as appropriate for the specific experiment.
Working with or transferring toxic chemicals in reaction flasks, separatory funnels, etc. Moderate
Broken or breaking glass during laboratory work/process (Injury from slipping glass)
  • Check the glassware to ensure it is free of cracks, flaws or scratches.
  • Wear slip-resistant and/or cut-resistant gloves, safety glasses with side shields, and lab coat when washing glassware.
  • Wear safety glasses with side shield for transporting glassware outside the lab.
  • Wear a face shield or safety goggles as appropriate for the specific experiment.
Washing lab glassware, transporting to or from drying ovens, autoclaves, etc. Low
Breaking glass ampules Use ampoule breakers or triangular file to score the glass as appropriate. Working with pharmaceutical and analytical standards or other sealed chemical ampoules. Low
Inserting the inlet of an auto-pipetting device onto a glass pipette
  • Check for defective pipettes with cracked or chipped ends.
Dispensing liquid reagents in labs. Low
Cutting glass tubes
  • Purchase commercially available aspirator flasks with prefabricated stopped/Pyrex glass tubing assemblies.
  • Use a triangular file or diamond pencil for scoring/scratching a glass tube or visit your local glass shop for assistance (335-1337)
Preparing aspirator/vacuum flasks filtration equipment Low

Injury and Illness Prevention

  1. Do not handle broken glass with bare hands. Use appropriate cut-resistant gloves to handle broken glass.
  2. Use forceps, tongs, scoops, or other mechanical devices for removing or retrieving broken glass from the work area or a fume hood.
  3. A dustpan and brush should be used to clean up shards/small pieces of broken glass, Pasteur pipettes, or shards of glass.
  4. Do not remove any items from a sharps container.
  5. Substitute glassware with plastic ware, including desiccators, wherever possible.


The principal investigator (PI) or laboratory supervisor is responsible for providing the most appropriate training on safe handling of glassware to their staff and students. The PI or laboratory supervisor is also responsible for conducting an equipment-specific and process-specific risk assessment and implementing safe work practices to minimize injuries due to broken glass.

  1. All lab staff using glassware and other sharps should receive training in safe handling and disposal of sharps.
  2. Researchers should consult with their supervisor prior to introducing any new processes in their research lab.
  3. Researchers should exercise caution while handling and transporting glassware.
  4. Researchers are responsible for wearing appropriate PPE during the cleanup and disposal of broken glassware.
  5. Researchers should read and follow the manufacturer’s instructions when assembling specialized glass apparatus and equipment that potentially contains sharps (e.g. autoclaves, automated dishwashers, microtome tissue slicers, etc.).

Personal Protective Equipment (PPE)

Equipment-specific safety precautions should be followed to prevent broken glass injuries by selecting and using proper PPE. Process-specific PPE may include cut-resistant gloves, full length lab coats/gowns, and a face shield/safety goggles.  These provide protection of the skin, including forearms, fingers, and the eyes. All research labs should have cut/puncture-resistant gloves similar to Kevlar® or D-Flex® and an approved sharps disposal container large enough to contain broken glass.

First Aid, Medical Attention, and Treatment

A tetanus shot is strongly encouraged for laboratory researchers who handle glassware activities such as cleaning and removal of glassware from hot ovens or autoclaves, etc. In the event of an injury involving a broken glass or sharp instrument, the following steps should be followed:

  1. Encourage bleeding of the wound; but do not directly massage or attempt other forceful means to remove any remaining broken glass from the wound.
  2. Wash the wound thoroughly with warm water and soap if available. Use cold water if warm water is not available.
  3. Seek medical attention as appropriate. Detailed emergency information is available at the EHS web site for medical attention and emergency treatment.
  4. Immediately report the incident to the PI or lab supervisor, as all injuries from lab incidents exposures and near miss incidents should be reported to these individuals.
  5. If the incident involves potential exposure to infectious materials, then immediate medical attention is required. In situations where the risk of exposure to infectious materials is unknown or uncertain, and the injury occurred in a biological research area, the injury should be treated as a possible exposure to infectious material and reported immediately to the Associate Biological Safety Officer.


Written by Ogunsemore Anthony

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