HPLC Basics – principles and parameters
PRINCIPLE OF HPLC Fig. 1 Schematic layout of a HPLC system The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent)…
PRINCIPLE OF HPLC Fig. 1 Schematic layout of a HPLC system The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent)…
High performance liquid chromatography (HPLC) is very popular and widely used in the pharmaceutical industry but is also an important tool for the analysis of samples hydrocarbon processing industry. State-of-the-art…
HPLC system care and troubleshooting is an important activity in any analytical chemistry lab. Liquid chromatography systems have become real workhorses for laboratory chemical analysis, but scientists have developed a…
Even the most carefully executed experiment can give erroneous results if dirty glassware is bought to use. If the glassware that is used for measuring liquids is contaminated with grease…
Almost every laboratory uses chemicals and reagents in one form or another. Knowing where to buy them, what grade to purchase, and what labeling is required can be confusing. This…
What is Biosafety: The application of knowledge, techniques and equipment to prevent personal, laboratory and environmental exposure to potentially infectious agents or biohazards. Biosafety defines the containment conditions under which…
If you have experienced retention time shifts with LC, you know it can be complicated and can cause problems with quantitation if not resolved. Hopefully this post will help you…
Nitrogen generators use a supply of dry, oil-free air to purify nitrogen via a selectively permeable membrane or carbon molecular sieve material which removes oxygen, carbon monoxide and carbon dioxide.…
While you can drink tap water, it's not suitable for most laboratory tests, preparing solutions, calibrating equipment, or cleaning glassware. For the lab, you want purified water. Common purification methods…
In the last decade, inductively coupled plasma triple quadrupole mass spectrometry (ICP-QQQ), also called inductively coupled plasma tandem mass spectrometry (ICP-MS/MS), has been a game changer in the world of…
UV Lamps have been there for more than a century. Apart from usual lighting applications, these lamps have been used in a variety of utilities that can be accounted as…
Laboratory workers and researchers must be cognizant that laboratory equipment can malfunction without adequate monitoring and regularly scheduled maintenance. Equipment typically does not undergo catastrophic failures without earlier indications or…
The idea of this article is to cover working aspect of Hollow Cathode Lamps followed by its various advantages that fulfills Hollow Cathode Lamps’ usage in AAS. Working of Hollow…
The procedure for the sampling and analysis of soils for volatile organic compounds (TPH GRO, BTEX, MTBE and naphthalene by GC, and volatiles by GC/MS) was updated by the USEPA…
Unplanned and unexpected instrument downtime in a lab means delays and frustrations that can potentially damage a lab’s reputation to reliably deliver high-quality results. Problem: Unplanned and unexpected instrument downtime in a…